Alexander Frey

Peer-reviewed scientific articles

Journal article-refereed, Original research

Screening for novel genes of Saccharomyces cerevisiae involved in recombinant antibody production.

De Ruijter, Jorg; Jurgens, German; Frey, Alexander

2017 in FEMS YEAST RESEARCH (Wiley-Blackwell)

ISSN: 1567-1356

The trans-10,cis-12 conjugated linoleic acid increases triacylglycerol hydrolysis in yeast Saccharomyces cerevisiae

Hokkanen, Sanna; Laakso, Simo; Senn, C. M. ; Frey, A. D.

2017 in JOURNAL OF APPLIED MICROBIOLOGY (BLACKWELL PUBLISHING)

ISSN: 1364-5072

Following nature's roadmap folding factors from plasma cells led to improvements in antibody secretion in S. cerevisiae

Koskela, Essi V.; de Ruijter, Jorg C.; Frey, Alexander D.

2017 in BIOTECHNOLOGY JOURNAL (Wiley-VCH Verlag)

ISSN: 1860-6768

Enzymatic synthesis of fucose-containing galacto-oligosaccharides using β-galactosidase and identification of novel disaccharide structures

Usvalampi, Anne; Maaheimo, Hannu; Tossavainen, Olli; Frey, Alexander D.

2017 in GLYCOCONJUGATE JOURNAL (Springer Netherlands)

ISSN: 0282-0080

Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

de Ruijter, Jorg C.; Koskela, Essi V.; Frey, Alexander D.

2016 in MICROBIAL CELL FACTORIES (BIOMED CENTRAL LTD)

ISSN: 1475-2859

A dual approach for improving homogeneity of a human-type N-glycan structure in Saccharomyces cerevisiae

Piirainen, Mari A.; Boer, Harry; de Ruijter, Jorg C.; Frey, Alexander D.

2016 in GLYCOCONJUGATE JOURNAL (Springer Netherlands)

ISSN: 0282-0080

Analysis of antibody production in Saccharomyces cerevisiae: effects of ER protein quality control disruption

de Ruijter, Jorg C.; Frey, Alexander D.

2015 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (SPRINGER)

ISSN: 0175-7598

An enzyme-based screening system for the rapid assessment of protein N-glycosylation efficiency in yeast

Frey, Alexander D.; Aebi, Markus

2015 in GLYCOBIOLOGY (OXFORD UNIV PRESS INC)

ISSN: 0959-6658

Homologous Recombinatorial Cloning Without the Creation of Single-Stranded Ends Exonuclease and Ligation-Independent Cloning (ELIC)

Koskela, Essi; Frey, Alexander

2015 in MOLECULAR BIOTECHNOLOGY (HUMANA PRESS INC)

ISSN: 1073-6085

Protein degradation corrects for imbalanced subunit stoichiometry in OST complex assembly

Mueller, Susanne; Wahlander, Asa; Selevsek, Nathalie; Otto, Claudia; Ngwa, Elsy Mankah; Poljak, Kristina; Frey, Alexander D.; Aebi, Markus; Gauss, Robert

2015 in MOLECULAR BIOLOGY OF THE CELL (AMER SOC CELL BIOLOGY)

ISSN: 1059-1524

Glycoengineering of yeasts from the perspective of glycosylation efficiency

Piirainen, Mari A.; de Ruijter, Jorg C.; Koskela, Essi V.; Frey, Alexander D.

2014 in NEW BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 1871-6784

A combined system for engineering glycosylation efficiency and glycan structure in Saccharomyces cerevisiae

Nasab, Farnoush Parsaie; Aebi, Markus; Bernhard, Gesche; Frey, Alexander Daniel

2013 in APPLIED AND ENVIRONMENTAL MICROBIOLOGY (AMER SOC MICROBIOLOGY)

ISSN: 0099-2240

Genetic engineering of the pancreatic β-cell line MIN6 to express bacterial globin proteins protects cells from nitrosative stress

Frey, Alexander D.; Schmid, Vinzenz H.; Kallio, Pauli T.

2011 in Biotechnology and Applied Biochemistry (Wiley-Blackwell)

ISSN: 0885-4513

Reduction of N-linked xylose and fucose by expression of rat β1,4-N-acetylglucosaminyltransferase III in tobacco BY-2 cells depends on Golgi enzyme localization domain and genetic elements used for expression

Karg, Saskia R.; Frey, Alexander D.; Kallio, Pauli T.

2010 in JOURNAL OF BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 0168-1656

Expression of rat β(1,4)-N-acetylglucosaminyltransferase III in Nicotiana tabacum remodels the plant-specific N-glycosylation

Frey, Alexander D.; Karg, Saskia R.; Kallio, Pauli T.

2009 in PLANT BIOTECHNOLOGY JOURNAL (WILEY-BLACKWELL)

ISSN: 1467-7644

Intrinsic non-symbiotic and truncated haemoglobins and heterologous Vitreoscilla haemoglobin expression in plants

Jokipii-Lukkari, Soile; Frey, Alexander D.; Kallio, Pauli T.; Häggman, Hely

2009 in JOURNAL OF EXPERIMENTAL BOTANY (Oxford University Press)

ISSN: 0022-0957

A small-scale method for the preparation of plant N-linked glycans from soluble proteins for analysis by MALDI-TOF mass spectrometry

Karg, Saskia R.; Frey, Alexander D.; Ferrara, Claudia; Streich, Daniel K.; Umaña, Pablo; Kallio, Pauli T.

2009 in PLANT PHYSIOLOGY AND BIOCHEMISTRY (Elsevier Masson SAS)

ISSN: 0981-9428

Transcriptional activity of Pseudomonas aeruginosa fhp promoter is dependent on two regulators in addition to FhpR

Koskenkorva, Taija; Aro-Kärkkäinen, Niina; Bachmann, Daniel; Arai, Hiroyuki; Frey, Alexander D.; Kallio, Pauli T.

2008 in ARCHIVES OF MICROBIOLOGY (Springer-Verlag)

ISSN: 0302-8933

Analysis of the contribution of the globin and reductase domains to the ligand-binding properties of bacterial haemoglobins

Farrés, Judith; Burckhardt-Herold, Susanna; Scherrer, Jan; Frey, Alexander D.; Kallio, Pauli T.

2007 in BIOCHEMICAL JOURNAL (PORTLAND PRESS LTD)

ISSN: 0264-6021

Globin-expression postpones onset of stationary phase specific gene expression in Escherichia coli

Frey, Alexander D.; Andersson, Charlotte I J; Schmid, Vinzenz H.; Bülow, Leif; Kallio, Pauli T.

2007 in JOURNAL OF BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 0168-1656

WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cζ

Fritzius, Thorsten; Frey, Alexander D.; Schweneker, Marc; Mayer, Daniel; Moelling, Karin

2007 in FEBS JOURNAL (BLACKWELL PUBLISHING)

ISSN: 1742-464X

Analysis of novel hemoglobins during microaerobic growth of HMP-negative Escherichia coli

Kallio, Pauli T.; Heidrich, Jaqueline; Koskenkorva, Taija; Bollinger, C. J T; Farrés, Judith; Frey, Alexander D.

2007 in ENZYME AND MICROBIAL TECHNOLOGY (BUTTERWORTH-HEINEMANN)

ISSN: 0141-0229

A WD-FYVE protein binds to the kinases Akt and PKCζ/λ

Fritzius, Thorsten; Burkard, Gabriela; Haas, Elvira; Heinrich, Jochen; Schweneker, Marc; Bosse, Magnus; Zimmermann, Sven; Frey, Alexander D.; Caelers, Antje; Bachmann, Andre S.; Moelling, Karin

2006 in BIOCHEMICAL JOURNAL (PORTLAND PRESS LTD)

ISSN: 0264-6021

Characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in Escherichia coli

Koskenkorva, Taija; Frey, Alexander D.; Kallio, Pauli T.

2006 in JOURNAL OF BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 0168-1656

Expression of Vitreoscilla hemoglobin improves the metabolism of xylose in recombinant yeast Saccharomyces cerevisiae under low oxygen conditions

Ruohonen, Laura; Aristidou, Aristos; Frey, Alexander D.; Penttilä, Merja; Kallio, Pauli T.

2006 in ENZYME AND MICROBIAL TECHNOLOGY (BUTTERWORTH-HEINEMANN)

ISSN: 0141-0229

Ligand binding properties of bacterial hemoglobins and flavohemoglobins

Farrés, Judith; Rechsteiner, Markus P.; Herold, Susanna; Frey, Alexander D.; Kallio, Pauli T.

2005 in BIOCHEMISTRY (AMER CHEMICAL SOC)

ISSN: 0006-2960

Nitric oxide detoxification - a new era for bacterial globins in biotechnology?

Frey, Alexander D.; Kallio, Pauli T.

2005 in TRENDS IN BIOTECHNOLOGY (ELSEVIER SCIENCE LONDON)

ISSN: 0167-7799

Expression of Vitreoscilla haemoglobin in tobacco cell cultures relieves nitrosative stress in vivo and protects from NO in vitro

Frey, Alexander D.; Oberlet, Bettina T.; Farrés, Judith; Kallio, Pauli T.

2004 in PLANT BIOTECHNOLOGY JOURNAL (WILEY-BLACKWELL)

ISSN: 1467-7644

Bacterial hemoglobins and flavohemoglobins Versatile proteins and their impact on microbiology and biotechnology

Frey, Alexander D.; Kallio, Pauli T.

2003 in FEMS MICROBIOLOGY REVIEWS (Elsevier Science B.V.)

ISSN: 0168-6445

Vitreoscilla hemoglobin promoter is not responsive to nitrosative and oxidative stress in Escherichia coli

Frey, Alexander D.; Koskenkorva, Taija; Kallio, Pauli T.

2003 in FEMS MICROBIOLOGY LETTERS (Elsevier Science B.V.)

ISSN: 0378-1097

Expression of Vitreoscilla haemoglobin in hybrid aspen (Populus tremula x tremuloides)

Häggman, Hely; Frey, Alexander D. ; Ryynänen, Leena; Aronen, Tuija; Julkunen-Tiitto, Riitta; Tiimonen, Heidi; Pihakaski-Maunsbach, Kaarina; Jokipii, Soile; Chen, Xiwen; Kallio, Pauli T.

2003 in PLANT BIOTECHNOLOGY JOURNAL (WILEY-BLACKWELL)

ISSN: 1467-7644

Bacterial hemoglobins and flavohemoglobins for alleviation of nitrosative stress in Escherichia coli

Frey, Alexander D.; Farrés, Judith; Bollinger, C. J T; Kallio, Pauli T.

2002 in APPLIED AND ENVIRONMENTAL MICROBIOLOGY (AMER SOC MICROBIOLOGY)

ISSN: 0099-2240

Novel pristinamycin-responsive expression systems for plant cells

Frey, Alexander D.; Rimann, Markus; Bailey, James E.; Kallio, Pauli T.; Thompson, Charles J.; Fussenegger, Martin

2001 in BIOTECHNOLOGY AND BIOENGINEERING (Wiley-Blackwell)

ISSN: 0006-3592

Dissection of central carbon metabolism of hemoglobin-expressing Escherichia coli by 13C nuclear magnetic resonance flux distribution analysis in microaerobic bioprocesses

Frey, A. D.; Fiaux, J.; Szyperski, T.; Wüthrich, K.; Bailey, J. E.; Kallio, P. T.

2001 in APPLIED AND ENVIRONMENTAL MICROBIOLOGY (AMER SOC MICROBIOLOGY)

ISSN: 0099-2240

Expression of Alcaligenes eutrophus flavohemoprotein and engineered Vitreoscilla hemoglobin-reductase fusion protein for improved hypoxic growth of Escherichia coli

Frey, Alexander D.; Bailey, James E.; Kallio, Pauli T.

2000 in APPLIED AND ENVIRONMENTAL MICROBIOLOGY (AMER SOC MICROBIOLOGY)

ISSN: 0099-2240

Review article, Literature review, Systematic review

Chapter 3 – The Single-Domain Globin of Vitreoscilla: Augmentation of Aerobic Metabolism for Biotechnological Applications

Frey, Alexander D.; Shepherd, Mark; Jokipii-Lukkari, Soile; Häggman, Hely; Kallio, Pauli T.

2011 in ADVANCES IN MICROBIAL PHYSIOLOGY (Academic Press Inc.)

ISSN: 0065-2911

Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in E. coli

Kallio, Pauli T.; Bollinger, Christian J T; Koskenkorva, Taija; Frey, Alexander D.

2008 in METHODS IN ENZYMOLOGY (Academic Press Inc.)

ISSN: 0076-6879

Conference proceedings

From vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteins

Kallio, Pauli T.; Frey, Alexander D. ; Bailey, James E.

2001

ISBN: 978-90-481-5756-3

Patents and invention disclosures

Patent

IMPROVED GLYCOSYLATION OF PROTEINS IN HOST CELLS

Aebi, Markus; Nasab, Farnoush Parsaie; Frey, Alexander

2011

NOVEL TOOLS FOR THE PRODUCTION OF GLYCOSYLATED PROTEINS IN HOST CELLS

Helenius, Jonne; Neupert, Christine; Aebi, Markus; Nasab, Farnoush Parsaie; Frey, Alexander

2010

Host cells and methods for the cytoprotection from oxidative and nitrosative stress

Frey, Alexander; Kallio, Pauli T.

2007

Analysis of modifications in the ER protein quality control system on heterologous protein expression in Saccharomyces cerevisiae

de Ruijter, Jorg; Koskela, Essi; Frey, Alexander

2014 in NEW BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 1871-6784

Genetic engineering of Saccharomyces cerevisiae for efficient IgG-assembly and secretion

Koskela, Essi; Frey, Alexander

2014 in NEW BIOTECHNOLOGY (Elsevier Science B.V.)

ISSN: 1871-6784

A synthetic N-glycosylation pathway in yeast

Frey, Alexander

2013

The sweet side of bitter pills: What can glycoengineering do for better therapeutic proteins?

Frey, Alexander

2013

Humanized yeast N-glycans

Frey, Alexander; Nasab, Farnoush Parsaie; Bernhard, G.

2013

The main future challenges in microbial biotechnology. Towards designer products

Frey, Alexander

2011

Optimization of expression hosts: From metabolic engineering to systems biotechnology

Frey, Alexander

2011

Glycoengineering for the production of therapeutic proteins

Frey, Alexander

2011

Measuring protein turnover by dynamic stable isotope labeling and targeted mass spectrometry

Frey, Alexander

2011

Small scale analysis of plant N-linked glycans by MALDI-TOF MS

Karg, S. R.; Ferrara, C.; Frey, A.

2006

The impact of globin proteins on pancreatic beta-cell survival

Rickert, S.; Schmid, V.; Wielinga, P.; Frey, Alexander

2006

Expression of Vhb-gene in hybrid aspen and cryostorage of the valuable lines

Jokipii, S.; Aronen, T.; Frey, A.D.; Ryynänen, L.; Julkunen-Tiitto, R.; Tiimonen, H.; Pihakaski-Maunsbach, K.; Kallio, P.T.; Häggman, H.

2003