Christine Schmidt

Overview

Genome Stability Lab (GSL)

Research in the group aims to better understand how ubiquitylation pathways regulate the DNA damage response and associated processes. To achieve this goal we are integrating cell biology, biochemistry, bioinformatics and high through-put/high-content quantitative microscopy/microarray techniques. The importance of ubiquitylation and DNA damage response pathways is illustrated by the fact that a declining ubiquitin system and accumulating DNA damage can give rise to various human disorders such as cancer and neurodegenerative diseases.

Publications

Cabello-Lobato MJ, Wang S, Schmidt CK. SAMHD1 sheds moonlight on DNA double-strand break repair. _ Trends Genetics _ (2017) (in press) doi: 10.1016/j.tig.2017.09.007.

Schmidt CK_$, Galanty Y$, Sczaniecka-Clift M, Coates J, Jhujh S, Demir M, Jackson SP. Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair. **_Nature Cell Biology**  (2015) 17: 1458-1470. $Co-first authors

Aymard F, Bugler B, Schmidt CK, Guillou E, Caron P, Briois S, Iacovoni JS, Daburon V, Miller KM, Jackson SP et al. Transcriptionally active chromatin recruits homologous recombination at DNA double-strand breaks. Nat Struct Mol Biol (2014) 21: 366–374.

Knobel PA, Belotserkovskaya R, Galanty Y, Schmidt CK, Jackson SP, Stracker TH. USP28 is recruited to sites of DNA damage by the tandem BRCT domains of 53BP1 but plays a minor role in double-strand break metabolism. Mol Cell Biol (2014) 34: 2062–2074.

Xi W_$, Schmidt CK$, Sanchez S, Gracias DH, Carazo-Salas RE, Butler R, Lawrence N, Jackson SP, and Schmidt OG. Molecular Insights into Division of Single Human Cancer Cells in On-Chip Transparent Microtubes. _ ACS Nano _ (2016) 10: 5835–5846. $ Co-corresponding authors; h ighlighted in acs.org (ACS, _June 2016) and sciencenews.org (_Sciencenews, _June 2016).

Xi W, Schmidt CK_$, Sanchez S$, Gracias DH, Carazo-Salas RE, Jackson SP, Schmidt OG. Rolled-up Functionalized Nanomembranes as Three-Dimensional Cavities for Single Cell Studies. **_Nano Letters** (2014) 14: 4197–4204. $Co-corresponding authors (Impact Factor: 13; Top 4 Nanoscience & Nanotechnology Journal by Impact Factor); featured as front cover image (_Cover Art)._

Koch B, Sanchez S_$, Schmidt CK$, Swiersy A, Jackson SP, Schmidt OG. Confinement and Deformation of Single Cells and Their Nuclei Inside Size-Adapted Microtubes. **_Adv Healthc Mater** (2014) 3: 1753–1758. $Co-corresponding authors; featured as back cover image.

Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T. Spatial dynamics of chromosome translocations in living cells. Science (2013) 341: 660–664.

Schmidt CK and Jackson SP. On your MARK, get SET(D2), go! H3K36me3 primes DNA mismatch repair. Cell (2013) 153: 513-515.

Smith EJ, Xi W, Makarov D, Monch I, Harazim S, Quinones VAB, Schmidt CK, Mei YF, Sanchez S, Schmidt OG. Lab-in-a-tube: ultracompact components for on-chip capture and detection of individual micro-/nanoorganisms. Lab Chip (2012) 12: 1917-1931.

Harazim SM, Xi W, Schmidt CK, Sanchez S, Schmidt OG. Fabrication and applications of large arrays of multifunctional rolled-up SiO/SiO2 microtubes. J Mater Chem (2012) 22: 2878-2884.

Schneede A, Schmidt CK, Holtta-Vuori M, Heeren J, Willenborg M, Blanz J, Domanskyy M, Breiden B, Brodesser S, Landgrebe J et al. Role for LAMP-2 in endosomal cholesterol transport. J Cell Mol Med (2011) 15: 280-295.

Roukos V, Misteli T, Schmidt CK. Descriptive no more: the dawn of high-throughput microscopy. Trends Cell Biol (2010) 20: 503-506.

Schulze S, Huang GS, Krause M, Aubyn D, Quinones VAB, Schmidt CK_$, Mei YF$, Schmidt OG. Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled-Up Nanotechnology. **_Adv Eng Mater** (2010) 12: B558-B564. $Co-corresponding authors

Schmidt CK, Brookes N, Uhlmann F. Conserved features of cohesin binding along fission yeast chromosomes. Genome Biol (2009) 10: R52.

Bernard P_$, Schmidt CK$, Vaur S$, Dheur S, Drogat J, Genier S, Ekwall K, Uhlmann F, Javerzat JP. Cell-cycle regulation of cohesin stability along fission yeast chromosomes. **_Embo J** (2008) 27: 111-121 $Co-first authors

D''Ambrosio C, Schmidt CK, Katou Y, Kelly G, Itoh T, Shirahige K, Uhlmann F. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev (2008) 22: 2215-2227.

Willenborg M, Schmidt CK, Braun P, Landgrebe J, von Figura K, Saftig P, Eskelinen, E-L. Mannose 6-phosphate receptors, Niemann-Pick C2 protein, and lysosomal cholesterol accumulation. J Lipid Res (2005) 46: 2559–2569.

Eskelinen E_$, Schmidt CK$, Neu S, Willenborg M, Fuertes G, Salvador N, Tanaka Y, Lüllmann-Rauch R, Hartmann D, Heeren J et al. Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts. **_Mol Biol Cell** (2004) 15: 3132–3145. $Co-first authors

Biography

Christine completed postdoctoral positions in Prof. Stephen Jackson''s group at the Gurdon Institute/University of Cambridge between 2011 and 2016, and Dr Tom Misteli''s group at the National Cancer Institute, National Institutes of Health, USA, between 2009 and 2011. She was awarded her PhD in Dr Frank Uhlmann''s group at the CRUK London Research Institute/University College London where she performed her studies between 2004 and 2009.