Anil Bamezai

Education:

• B.S. Degree College Udhampur, Jammu University, India 1979
• M.S. Jammu University, India 1982
• Ph.D. All India Institute of Medical Sciences, India 1987
• Post-Doctoral. Harvard Medical School, USA 1987-1990

Publications:

• Rathbun, LA., Magliocco, AM., and Bamezai. AK. 2023. Human LY6 gene family: potential tumor-associated antigens and biomarkers of prognosis in uterine corpus endometrial carcinoma. Oncotarget. 14:426-437.
• Patel, AG., Moxham, S., and Bamezai, AK. 2023. Ly-6A-Induced Growth Inhibition and Cell Death in a Transformed CD4+ T Cell Line: Role of Tumor Necrosis Factor-a. Archivum Immunologiae et Therapiae Experimentalis (Springer Publishing). 71, 4
• Bamezai AK and Miwa JM. 2022. Biology of Ly-6 Supergene Family in Health and Disease. Front. Cell Dev. Biol. 10:949379.
• Sengupta, S., Karsalia, R., Morrissey, A., and Bamezai AK. 2021.Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells. Scientific Reports (Nature Publishing). 11: 13970.
• Harris, E., Zimmerman, Devon., Warga, Eric., Bamezai, A., and Elmer, J. 2021. Non-Viral Gene Delivery to T Cells with Lipofectamine LTX. Biotechnol Bioeng. 118:1693-1706.
• Lang, MA., Jenkins,SA., Balzano, P., *Owoyele, A., *Patel, A., and Bamezai, AK. 2017 Engaging Ly-6A/Sca-1 triggers lipid raft-dependent and -independent responses in CD4+ T-cell lines. Immunity, Inflammation and Disease 5 (4): 448-460. Online: 28 JUN 2017, DOI: 10.1002/iid3.182
• Jones, M.,DeWolf, S., Vacharathit, V., Yim M., Spencer, S., and Bamezai, AK. 2016. Investigating B cell development, natural and primary antibody responses in Ly-6A/Sca-1 deficient mice. PLoS ONE, 11(6):e0157271.
• Comber, J.D and Bamezai, A. 2015. Gold Nanoparticles (AuNPs): A New Frontier in Vaccine Delivery. Journal of Nanomedicine Biotherapeutic Discov., 5:4 (Invited Editorial)
• Schieffer D, Naware S, Bakun W and Bamezai, AK. 2014. Lipid raft-based membrane order is important for antigen specific clonal expansion of CD4+ T lymphocytes. BMC Immunology, 15:58 (December 14, 2014). An "Editor's pick" article.
• Bamezai AK and Divakar Lal 2014. Self-assembling nanoparticle: A strategy for designing universal flu vaccine. Journal of Nanomedicine and Biotherapeutic Discovery 4 (2): e129 (Invited Editorial)
• Comber J.D., and Bamezai A. 2012. In vitro derivation of interferon-gamma producing, IL-4 and IL-7 responsive memory-like CD4+ T cells. Vaccine, 30(12):2140-2145
• Kennedy, C. Nelson, M. D. and Bamezai A. 2011. Analysis of Detergent-free Lipid Rafts isolated from a CD4+ T cell line: Interaction with antigen presenting cells promotes coalescing of lipid rafts. Cell Communication & Signaling. 9:31 (December 8, 2011)
• Bamezai, A. 2008. "Membrane rafts and Signaling". Immunology, Endocrinology and Metabolic Agents in Medicinal Chemistry, (Invited Editorial). 8:325-326
• Bamezai, A., Kennedy, C. 2008. Cell-free antibody capture method for analysis of detergent-resistant membrane rafts. Methods in Molecular Biology. 477: 137-147
• Reed,J.,Branigan, P., and Bamezai, A. 2008. Interferon-gamma enhances clonal expansion and survival of CD4+ T cells. Journal of Interferon and Cytokine Research. 28:611-622
• George, S., Nelson, M.D., Dollahon, N. and Bamezai, A. 2006. A novel approach to examining compositional heterogeneity of detergent-resistant lipid rafts. Immunology and Cell Biology. 84:192-202.
• Srinidhi Jayasuryan, Anil Bamezai and Vijay Gehlot. 2006. Petri-net based model of T cell receptor signaling pathway. Proceedings of 2006 International Conference on Bioinformatics & Computational Biology, BIOCOMP '06. (eds) Hamid R. Arabnia and Homayoun Valafar. p56-62.
• Bamezai, A. 2004. Mouse Ly-6 proteins and their extended family: markers of differentiation and regulators of cell signaling. Archives of Immunology and Experimental Therapy. 52 (4): 255-266. (Review article)
• Graduate student Undergraduate student

Research:

• T lymphocyte biology and Tumor Immunology: T lymphocytes express specific antigen receptors and are genetically geared to recognize internal (cancer) or external (pathogen) threats to the body. T cell activation is the first step in generating threat-specific T cell immune response. Activated T cells then rally other immune cells to generate an effective immunity. T cell activation and the responses it orchestrates is highly regulated to ensure no bodily harm caused in battling the threat. Inhibitory proteins expressed on the activated T cells are central in preventing excessive activation and responses generated by T cells. Ly-6A protein, a member of Ly-6 supergene family, contributes to self-regulation of CD4+ Helper T lymphocyte responses in mice. Ly-6A protein shows either no or low expression in naïve CD4+ T cells, its expression is highly upregulated upon T cell activation and in the presence of cytokines (IL-27 and IFN-gamma). In vitro and ex vivo studies provide strong evidence for Ly-6A as T cell inhibitory protein. Our laboratory is currently examining the immune checkpoint inhibitory role of Ly-6A using tumor (B16-F10 melanoma and MC38 adenocarcinoma) transplantation mouse models for potential cancer immunotherapy using Ly-6A-targeted antibody blockade strategy. Additionally, we are investigating how Ly-6A signals to cell interior in the absence of its transmembrane and cytoplasmic tail. Another project in our laboratory concerns the spatiotemporal aspect of cell signaling in CD4+ helper T lymphocytes with the focus on cholesterol-rich membrane nanodomains, also known as lipid rafts. Lipid rafts show high representation of sphingolipids, glycosyl-phosphatidylinsitol (GPI)-anchored proteins (e.g., Ly-6 proteins) and several lipid-modified signaling molecules. These membrane nanodomains are compositionally heterogeneous, dynamic and exist as 10-100 nanometer size structures in a variety of cell types. We have reported that these lipid nanodomains coalesce during interactions between CD4+ T cells with antigen presenting cells. We are currently investigating the role of cholesterol-rich membrane domains in T cell signaling and determining which T cell signal(s) regulate membrane lipid nanodomain coalescence. Additionally, we are examining the role of these membrane nanodomains in delivering signals generated after engaging tail-less GPI-anchored proteins (e.g., Ly-6A).

Professional Experience:

• President, VU Chapter Sigma Xi, 2011-12; Member, Education Committee, The American Association of Immunologists, 2016-2019; Member, Undergraduate Immunology Curriculum Development Sub-Committee, The American Association of Immunologists, 2019-2021; Guest Associate Editor, Frontiers in Cell and Developmental Biology - Signaling; Associate Editor, Frontiers in Immunology -T cell Biology.