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ICTD International Centre for Training and Development

Microbiological Methods for Examination of Water & Wastewater

Available dates

Dec 15—19, 2019
5 days
Dubai, United Arab Emirates
USD 3300
USD 660 per day

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About the course

Upon successful completion of this course, the delegates will be able to:

  • Examine wastewater for biological characteristics, identify various organisms, perform particle size and number counts, and advise the operators of a wastewater treatment plant on the implications of the microbiological information developed
  • Understand how the changes in microbiological populations in a wastewater treatment plant can affect the plant performance and what to do to bring it back into the proper operation
  • Participants will also be able to use JNOPTIC Capture 2.2 software

Agenda

Day 1

  • Introduction to Microscopy
  • Basic Microscopy
  • Introduction to Microscopy
  • Different types of Microscopy
  • Bright Field Microscopy
  • Cross-Polarized Light Microscopy
  • Dark Field vs. Bright Field
  • Variants on Dark Field Microscopy
  • Examples of Rheinberg Darkfield Microscopy
  • Kohler Illumination Microscopy
  • EPI Illumination
  • Phase Contrast Microscopy Theory and Practice

Day 2

  • Basics Taxonomy
  • Order of Biology
  • Kingdom-Phylum- Class –Order –Family –Genus -Species
  • Types of Microorganisms:

Bacteria

Protozoa: (including Guardia and Cryptosporidium)

Metazoa: (Multicellular organisms)

Viruses

Important Terms

Parasites and Pathogens: worms and fungi

Soil Microbes and Heterotrophs: Bacteria; protozoans; nematodes; Obligate aerobes; Anaerobes; facultative organisms

  • Bacteria Characteristics and Identification of types
  • Size & Shapes Sheathed; Stalked; Budding; Filamentous; Cyanobateria

  • Parts of the Cell and Identification

  • Introduction to Dye Staining

  • Gram Positive vs Gram Negative

  • Dye staining techniques

  • Gram Negative Bacteria Flowchart

  • Components of a bacterial cell

  • Extracellular proteins

  • Flagella and Pili Motile bacteria

  • Inclusions and vacuoles

  • Endospores

  • Autotrophs Heterotrophs

  • Photoautotrophs

  • Chemolithrophs

  • Aerobic and Anaerobic Bacteria: Psychrophilic; Mesophilic and Thermophilic Bacteria Typical Growth Patterns; Time and concentration for growth

  • Protozoa Identification stramennopiles protozoa, fungi and plants

  • Amoebae: Ciliates, Common Wastewater Ciliates, Rotifers, Wastewater Rotifers

  • Rotifer Identification

  • Nematodes & Roundworms

  • Using a Counting Chamber Hemocyometer

  • Other Metezoa Water bears and Water Fleas

  • Helminth Ova:

Some associated diseases

Day 3

Wastewater Microbiology Applications

The Activated Sludge Process

Detailed Walk through on local Wastewater Treatment Plant

  • Organotrophs aerobic or anaerobic, includes all animals and fungi, and many bacteria and protists.

Organotrophs' energy extraction

Wastewater Microbiology Applications Nitrifying bacteria

  • chemoautotrophs
  • chemolithotrophs:

Nitrosomonas, Nitrosococcus, Nitrobacter, Nitrococcus and their significance

Wastewater Microbiology Applications

Nitrification and denitrification

Population Dynamics

Day 4

Detailed Practices

FLAVOBACTERIUM

MICROBIOLOGICAL FLOC FORMATION

Types of problems:

  • Pinfloc
  • Filamentous Floc & Bulking
  • Rising Floc
  • Foaming

Factor affecting floc formation:

  • Clarifier Underflow/Sludge Wasting

Filamentous Organism and Wastewater Treatment

  • Tests and Procedures on Mixed Liquor in an AS System
  • Measuring Total Floc Length

Identification of filaments & Jenkins guide

Nocardia spp.Type 1701

Type 021N

Type 0041

Thiothrix spp.

Sphaerotilus natans

Type 0092

Haliscomenobacter hydrossis

Type 0675

Factors affecting growth of Filamentous Organisms:

  • MCRT
  • DO Concentration
  • Toxics (including chlorine)
  • pH
  • Sulfide

Filament Control methods

  • Selective Toxicity
  • Chlorination control
  • Hydrogen Peroxide
  • Ozone
  • nutrient additions vs. deficiencies
  • compounds to add
  • Foam and Scum Controls

Identification of Filamentous Bacteria

A Simple Approach

What’s Needed A listing of supplies

  • Separation by Staining
  • Graham and Neisser
  • Typical Observations Filament Shape
  • Filament Size
  • Cell shape
  • Cell size
  • Cell Septa
  • Indentations
  • Sheath
  • Branching and other properties

Beggiatoa

More on bulking and foaming and how to identify various organisms

Nocardia

Good Practice

  • Keep a process chart of treatment system parameters
  • Measure the parameters consistently and routinely
  • Measure the parameters when the system is running properly
  • Exercises and Class Problems

Day 5

Software & Applications:

  • Helminth Ova,
  • significance, & control: The art of microscopic photography, Counting Chamber use;

Software use

Lab Work

Who should attend

This course is designed for Laboratory staff, Chemist, Laboratory technicians, Environmental Specialist, Wastewater Treatment Plant Operators, Treatment plant engineers and anyone involved in microbiological analysis.

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