Microbiological Methods for Examination of Water & Wastewater
ICTD International Centre for Training and Development
How long?
- 5 days
- in person
What are the topics?
ICTD International Centre for Training and Development
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Essentials
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Full
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Who should attend
This course is designed for Laboratory staff, Chemist, Laboratory technicians, Environmental Specialist, Wastewater Treatment Plant Operators, Treatment plant engineers and anyone involved in microbiological analysis.
About the course
Upon successful completion of this course, the delegates will be able to:
- Examine wastewater for biological characteristics, identify various organisms, perform particle size and number counts, and advise the operators of a wastewater treatment plant on the implications of the microbiological information developed
- Understand how the changes in microbiological populations in a wastewater treatment plant can affect the plant performance and what to do to bring it back into the proper operation
- Participants will also be able to use JNOPTIC Capture 2.2 software
Agenda
Day 1
- Introduction to Microscopy
- Basic Microscopy
- Introduction to Microscopy
- Different types of Microscopy
- Bright Field Microscopy
- Cross-Polarized Light Microscopy
- Dark Field vs. Bright Field
- Variants on Dark Field Microscopy
- Examples of Rheinberg Darkfield Microscopy
- Kohler Illumination Microscopy
- EPI Illumination
- Phase Contrast Microscopy Theory and Practice
Day 2
- Basics Taxonomy
- Order of Biology
- Kingdom-Phylum- Class –Order –Family –Genus -Species
- Types of Microorganisms:
Bacteria
Protozoa: (including Guardia and Cryptosporidium)
Metazoa: (Multicellular organisms)
Viruses
Important Terms
Parasites and Pathogens: worms and fungi
Soil Microbes and Heterotrophs: Bacteria; protozoans; nematodes; Obligate aerobes; Anaerobes; facultative organisms
- Bacteria Characteristics and Identification of types
Size & Shapes Sheathed; Stalked; Budding; Filamentous; Cyanobateria
Parts of the Cell and Identification
Introduction to Dye Staining
Gram Positive vs Gram Negative
Dye staining techniques
Gram Negative Bacteria Flowchart
Components of a bacterial cell
Extracellular proteins
Flagella and Pili Motile bacteria
Inclusions and vacuoles
Endospores
Autotrophs Heterotrophs
Photoautotrophs
Chemolithrophs
Aerobic and Anaerobic Bacteria: Psychrophilic; Mesophilic and Thermophilic Bacteria Typical Growth Patterns; Time and concentration for growth
Protozoa Identification stramennopiles protozoa, fungi and plants
Amoebae: Ciliates, Common Wastewater Ciliates, Rotifers, Wastewater Rotifers
Rotifer Identification
Nematodes & Roundworms
Using a Counting Chamber Hemocyometer
Other Metezoa Water bears and Water Fleas
Helminth Ova:
Some associated diseases
Day 3
Wastewater Microbiology Applications
The Activated Sludge Process
Detailed Walk through on local Wastewater Treatment Plant
- Organotrophs aerobic or anaerobic, includes all animals and fungi, and many bacteria and protists.
Organotrophs' energy extraction
Wastewater Microbiology Applications Nitrifying bacteria
- chemoautotrophs
- chemolithotrophs:
Nitrosomonas, Nitrosococcus, Nitrobacter, Nitrococcus and their significance
Wastewater Microbiology Applications
Nitrification and denitrification
Population Dynamics
Day 4
Detailed Practices
FLAVOBACTERIUM
MICROBIOLOGICAL FLOC FORMATION
Types of problems:
- Pinfloc
- Filamentous Floc & Bulking
- Rising Floc
- Foaming
Factor affecting floc formation:
- Clarifier Underflow/Sludge Wasting
Filamentous Organism and Wastewater Treatment
- Tests and Procedures on Mixed Liquor in an AS System
- Measuring Total Floc Length
Identification of filaments & Jenkins guide
Nocardia spp.Type 1701
Type 021N
Type 0041
Thiothrix spp.
Sphaerotilus natans
Type 0092
Haliscomenobacter hydrossis
Type 0675
Factors affecting growth of Filamentous Organisms:
- MCRT
- DO Concentration
- Toxics (including chlorine)
- pH
- Sulfide
Filament Control methods
- Selective Toxicity
- Chlorination control
- Hydrogen Peroxide
- Ozone
- nutrient additions vs. deficiencies
- compounds to add
- Foam and Scum Controls
Identification of Filamentous Bacteria
A Simple Approach
What’s Needed A listing of supplies
- Separation by Staining
- Graham and Neisser
- Typical Observations Filament Shape
- Filament Size
- Cell shape
- Cell size
- Cell Septa
- Indentations
- Sheath
- Branching and other properties
Beggiatoa
More on bulking and foaming and how to identify various organisms
Nocardia
Good Practice
- Keep a process chart of treatment system parameters
- Measure the parameters consistently and routinely
- Measure the parameters when the system is running properly
- Exercises and Class Problems
Day 5
Software & Applications:
- Helminth Ova,
- significance, & control: The art of microscopic photography, Counting Chamber use;
Software use
Lab Work
Videos and materials
Microbiological Methods for Examination of Water & Wastewater at ICTD International Centre for Training and Development
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